rabbit anti tff3  (Proteintech)

Journal: Molecular Medicine Reports

Article Title: ST3Gal1 modulates intestinal barrier function and impacts human ulcerative colitis

doi: 10.3892/mmr.2025.13783

Figure Lengend Snippet: ST3Gal1 expression in inflamed IEC monolayers alters MUC2, TFF3, CDX2, STAT3 and p-STAT3 expression. ST3Gal1 knockdown in ST3Gal1-sh3/IEC significantly increased (A) MUC2, (B) TFF3 and (C) CDX2, but significantly reduced (D) STAT3 mRNA levels in the inflamed IEC monolayer. ST3Gal1 OE in ST3Gal1-OE/IEC significantly reduced (E) MUC2, (F) TFF3 and (G) CDX2 mRNA levels in the inflamed IEC monolayer. (H) ST3Gal1 OE in ST3Gal1-OE/IEC slightly decreased STAT3 mRNA levels in the inflamed IEC monolayer. (I) Western blotting showed no changes in protein expression in non-inflamed knockdown samples. (J) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were increased, and p-STAT3 expression was decreased in the inflamed IEC monolayer comprising ST3Gal1-sh3/IEC. (K) Western blotting showed no notable changes in protein expression in non-inflamed OE samples. (L) Western blotting showed that MUC2, TFF3 and CDX2 protein levels were decreased, and p-STAT3 expression was increased in the inflamed IEC monolayer comprising ST3Gal1-OE/IEC. Data are presented as the mean ± SD. Statistical significance was assessed using one-way analysis of variance followed by Tukey's post-hoc test. *P<0.05 and **P<0.01. IEC, intestinal epithelial cell; sh, short hairpin; Ctr, control; OE, overexpression; MUC2, mucin 2; TFF3, trefoil factor 3; CDX2, homeobox protein CDX-2; p-, phosphorylated.

Article Snippet: Rabbit Polyclonal IgG to TFF3 , Proteintech Group, Inc. , 23277-1-AP , 1:1,000.

Techniques: Expressing, Knockdown, Western Blot, Control, Over Expression

Journal: Molecular Medicine Reports

Article Title: ST3Gal1 modulates intestinal barrier function and impacts human ulcerative colitis

doi: 10.3892/mmr.2025.13783

Figure Lengend Snippet: Comparison of (A) MUC2, (B) TFF3 and (C) CDX2 mRNA levels between non-lesional mucosa (n=44) and lesional mucosa (n=75) from distinct patients with ulcerative colitis. Correlations between (D) MUC2, (E) TFF3 and (F) CDX2 mRNA levels and ST3Gal1 expression. Data in A-C are presented as box and whisker plots, and those in D-F as scatter plots. Statistical significance for group comparisons in A-C was assessed using unpaired t-test. Correlation analyses in D-F were performed using Pearson's correlation coefficient. *P<0.05 and **P<0.01. Data were obtained from the GSE107499 dataset from the Gene Expression Omnibus database. *P<0.05 and **P<0.01. NS, not significant; MUC2, mucin 2; TFF3, trefoil factor 3; CDX2, homeobox protein CDX-2.

Article Snippet: Rabbit Polyclonal IgG to TFF3 , Proteintech Group, Inc. , 23277-1-AP , 1:1,000.

Techniques: Comparison, Expressing, Whisker Assay, Gene Expression

Journal: Biology Open

Article Title: Src family kinases inhibit differentiation of intestinal epithelial cells through the Hippo effector YAP1

doi: 10.1242/bio.058904

Figure Lengend Snippet: The effect of SFK inhibition on the differentiation of absorptive and secretory cells in HT29 (left panels) and Caco-2/15 cells (right panels). (A) Transcript expression of the absorptive cell marker SI and goblet cell marker MUC2 in HT29 and absorptive cell markers SI and DPPIV in Caco-2/15 cells incubated with SFK inhibitors (PP2 and dasatinib) relative to control (DMSO). (B) Representative western blot analysis and data compilation showing higher expression of MUC2, TFF3, SI and DPPIV in PP2 and dasatinib treated HT29 and Caco-2/15 cells relative to DMSO treated cells. β-actin was used as a loading control. Results are expressed as mean±s.e.m.; n =3; * P <0.05, ** P <0.005, # P =0.063.

Article Snippet: In the present study, mouse monoclonal anti-MUC2 (ab11197, 1/500 WB and 1/100 IF, Abcam), rabbit monoclonal anti-TFF3 (ab108599, 1/1500 WB, Abcam), mouse monoclonal anti-SI ([53]; HSI-4/34 or Caco-3/73, 1/100 WB), rabbit monoclonal anti-DPPIV or CD26 [EPR20819] (ab215711, 1/2000 WB, Abcam), rabbit monoclonal anti-YAP/TAZ (D24E4, 1/1500 WB, Cell Signaling Technology, Danvers, MA, USA), rabbit monoclonal anti-YAP (D8H1X, 1/1000 WB, 1/150 IF, Cell Signaling Technology), mouse monoclonal anti-TAZ (M2-616, 1/300 IF, BD Biosciences, NJ, USA) mouse monoclonal anti-CDX2-CD88 (MU392A-UC, 1/700, BioGenex, Freemont, CA, USA), mouse monoclonal anti-HNF1α [F-7] (sc-393925, 1/300 WB, Santa Cruz Biotechnology, Dallas, TX, USA), goat polyclonal anti-HNF4α [C-19] (sc-6556, 1/600 WB, Santa Cruz Biotechnology) and anti-β-actin (MAB1501, 1/20,000, Millipore, Etobicoke, ON, Canada) were used as primary antibodies.

Techniques: Inhibition, Expressing, Marker, Incubation, Western Blot

Journal: bioRxiv

Article Title: Mitogen-activated protein kinase activity drives cell trajectories in colorectal cancer

doi: 10.1101/2020.01.10.901579

Figure Lengend Snippet: Immunofluorescence analysis for OLFM4, MKI67, FABP1, and TFF3 in normal and tumor tissue. All sections are from patient P009, except the EPCAM/OLFM4 co-staining that was done on tumor tissue of P016. Scale bars indicate 100μm. For marker expression in UMAP space, see Supplementary Fig. 3, above.

Article Snippet: Immunostainings were performed on the BenchMark XT immunostainer (Ventana Medical Systems), using CC1 mild buffer or Ultra CC1 buffer (Ventana Medical Systems) for 30 min at 100°C, and using antibodies rabbit anti-TFF3 (1:250, Abcam, ab108599), mouse anti-FABP1 (1:1000, Abcam, ab7366), rabbit anti-OLFM4 (1:100, Atlas Antibodies, HPA077718), mouse anti-EPCAM (1:100, ThermoScientific, MS-144-P1), rabbit anti-Ki67 (1:400, Abcam, ab16667), mouse anti-Ki67 (1:50, Dako, M7240), rabbit anti-LYZ (1:1500, Abcam, ab108508), rabbit anti-EREG (1:50, Thermo Fischer Scientific, PA5-24727), anti-PARP1, mouse anti-MUC2 (1:50, Leica, NCL-MUC-2), mouse anti-CK17 (1:10, Dako, M7046), mouse anti-MMP7 (1:100, ThermoFisherScientific, MA5-14215).

Techniques: Immunofluorescence, Staining, Marker, Expressing

Journal: bioRxiv

Article Title: Mitogen-activated protein kinase activity drives cell trajectories in colorectal cancer

doi: 10.1101/2020.01.10.901579

Figure Lengend Snippet: A Phenotypes of patient-derived organoid lines P009T and P013T. B UMAPs of organoid single cell transcriptomes. Organoid lines and medium conditions as indicated. LGR5-ISC stem cell, enterocyte and Goblet cell signatures are visualized. C Schematic representation of SLAM-Seq workflow to infer RNA dynamics (“RNA velocity”). In short, organoids were passaged and assigned to different medium conditions. After three days, nascent RNA was metabolically labelled for 2 h using 4sU. Organoids were harvested, dissociated, and fixed. RNA in single cells was alkylated, and cells were subjected to single cell sequencing. Reads were assigned to nascent or old RNA status, depending on diagnostic T-C mutational status. D Developmental trajectories inferred from RNA metabolic labelling. Bold and thin arrows indicate high versus low directionality of RNA velocity. Colors below RNA velocity show latent time (yellow: early latent time; blue: late latent time). E Activities of Wnt/β-catenin and MAPK target genes in organoid single cell transcriptomes, ordered along latent time. F Activities of TFF3 , FABP1 , MKI67 and MMP7 in organoid single cell transcriptomes, ordered along latent time. Color code for panels D-F: Red: high activity; blue: low activity.

Article Snippet: Immunostainings were performed on the BenchMark XT immunostainer (Ventana Medical Systems), using CC1 mild buffer or Ultra CC1 buffer (Ventana Medical Systems) for 30 min at 100°C, and using antibodies rabbit anti-TFF3 (1:250, Abcam, ab108599), mouse anti-FABP1 (1:1000, Abcam, ab7366), rabbit anti-OLFM4 (1:100, Atlas Antibodies, HPA077718), mouse anti-EPCAM (1:100, ThermoScientific, MS-144-P1), rabbit anti-Ki67 (1:400, Abcam, ab16667), mouse anti-Ki67 (1:50, Dako, M7240), rabbit anti-LYZ (1:1500, Abcam, ab108508), rabbit anti-EREG (1:50, Thermo Fischer Scientific, PA5-24727), anti-PARP1, mouse anti-MUC2 (1:50, Leica, NCL-MUC-2), mouse anti-CK17 (1:10, Dako, M7046), mouse anti-MMP7 (1:100, ThermoFisherScientific, MA5-14215).

Techniques: Derivative Assay, Metabolic Labelling, Sequencing, Diagnostic Assay, Activity Assay

Journal: Oncology Letters

Article Title: The expression of trefoil factor 3 is related to histologic subtypes and invasiveness in lung adenocarcinoma

doi: 10.3892/ol.2020.12325

Figure Lengend Snippet: Varying immunohistochemical staining intensities demonstrate TFF3 expression in normal lung and lung adenocarcinoma. (A) Non-cancerous alveolar epithelial cells hardly expressed TFF3. (B) Normal bronchial glands and bronchial epithelium strongly expressed TFF3. (C-E) Representative immunohistochemistry images of lung adenocarcinoma were presented with intensity scores of weak (1+; C), moderate (2+; D) and strong (3+; E), respectively. Scale bars in (A and B), 100 µm; scale bares in (C-E), 50 µm. TTF3, trefoil factor 3.

Article Snippet: Expression of TFF3 was examined with the primary rabbit anti-TFF3 monoclonal antibody (dilution 1:2,000 cat. no. ab108599; Abcam).

Techniques: Immunohistochemical staining, Staining, Expressing, Immunohistochemistry

Journal: Oncology Letters

Article Title: The expression of trefoil factor 3 is related to histologic subtypes and invasiveness in lung adenocarcinoma

doi: 10.3892/ol.2020.12325

Figure Lengend Snippet: TFF3 expression in various histologic subtypes of lung adenocarcinoma.

Article Snippet: Expression of TFF3 was examined with the primary rabbit anti-TFF3 monoclonal antibody (dilution 1:2,000 cat. no. ab108599; Abcam).

Techniques: Expressing

Journal: Oncology Letters

Article Title: The expression of trefoil factor 3 is related to histologic subtypes and invasiveness in lung adenocarcinoma

doi: 10.3892/ol.2020.12325

Figure Lengend Snippet: Trefoil factor 3 expression in various lung adenocarcinoma histologic subtypes. Typical images were presented in (A) lepidic adenocarcinoma, (B) acinar adenocarcinoma, (C) papillary adenocarcinoma, (D) solid adenocarcinoma and (E) invasive mucinous adenocarcinoma. Scale bars, 100 µm.

Article Snippet: Expression of TFF3 was examined with the primary rabbit anti-TFF3 monoclonal antibody (dilution 1:2,000 cat. no. ab108599; Abcam).

Techniques: Expressing

Journal: Oncology Letters

Article Title: The expression of trefoil factor 3 is related to histologic subtypes and invasiveness in lung adenocarcinoma

doi: 10.3892/ol.2020.12325

Figure Lengend Snippet: Varying TFF3 expression in an individual sample with mixed histologic subtypes. (A) Immunohistochemistry of TFF3 in a case with both lepidic and papillary areas. The boxed area with a solid line represents the lepidic area, which was enlarged in (B). The boxed area with a dotted line represents the papillary area, which was enlarged in (C). (D) The comparison of lepidic area and papillary/acinar area H-scores in 38 cases with both these areas. Each line represented the H-score of lepidic and papillary/acinar area of each case, and the data revealed a significant increase in papillary/acinar area from 22 of the 38 cases (red line). No differential expression in other samples was marked with a black line. The Wilcoxon signed-rank test was used to determine significance. Scale bar in (A), 1 mm; Scale bars in (B an C), 250 µm. TTF3, trefoil factor 3.

Article Snippet: Expression of TFF3 was examined with the primary rabbit anti-TFF3 monoclonal antibody (dilution 1:2,000 cat. no. ab108599; Abcam).

Techniques: Expressing, Immunohistochemistry

Journal: Oncology Letters

Article Title: The expression of trefoil factor 3 is related to histologic subtypes and invasiveness in lung adenocarcinoma

doi: 10.3892/ol.2020.12325

Figure Lengend Snippet: The association between TFF3 expression and adenocarcinoma lung cell invasiveness. (A) Confirmation of TFF3 knockdown in siTFF3 #1, #2 and #3 A549 cells. Equal protein loading was confirmed by quantifying β-actin (input control). TFF3 expression was quantified using ImageJ version 1.52 software. TFF3/β-actin quotient of control cells is expressed as 1. The relative quotient of TFF3-knockdown cells is presented as the ratio to that of control cells. (B) Matrigel invasion assay. Representative images of invading #1, #2 and #3 and control cells are presented. Invasive cells were counted in 5 random fields of view. Data are presented as the mean ± standard error. *P<0.05 and **P<0.01, as indicated. Scale bars, 100 µm. TTF3, trefoil factor 3; si, small interfering RNA.

Article Snippet: Expression of TFF3 was examined with the primary rabbit anti-TFF3 monoclonal antibody (dilution 1:2,000 cat. no. ab108599; Abcam).

Techniques: Expressing, Software, Invasion Assay, Small Interfering RNA